BAX Antibody, KO Validated
Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
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![Western blot analysis of extracts of various cell lines, using BAX antibody (14-223) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s. Western blot analysis of extracts of various cell lines, using BAX antibody (14-223) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.](https://www.prosci-inc.com/static-images/14-223_1.jpg)
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![Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody (14-223) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s. Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody (14-223) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.](https://www.prosci-inc.com/static-images/14-223_2.jpg)
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![Immunohistochemistry of paraffin-embedded rat ovary using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded rat ovary using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_3.jpg)
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![Immunohistochemistry of paraffin-embedded human lung cancer using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human lung cancer using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_4.jpg)
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![Immunohistochemistry of paraffin-embedded human breast cancer using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human breast cancer using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_5.jpg)
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![Immunohistochemistry of paraffin-embedded human stomach using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human stomach using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_6.jpg)
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![Immunohistochemistry of paraffin-embedded mouse liver using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse liver using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_7.jpg)
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![Immunohistochemistry of paraffin-embedded mouse kidney using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse kidney using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_8.jpg)
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![Immunohistochemistry of paraffin-embedded mouse heart using BAX antibody (14-223) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse heart using BAX antibody (14-223) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_9.jpg)
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![Immunohistochemistry of paraffin-embedded mouse brain using BRCA1 antibody (14-048) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse brain using BRCA1 antibody (14-048) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/14-223_10.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IP: 1:50 - 1:200
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX (NP_620116.1).
581
BCL2-associated X protein
BAX
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Affinity purification
Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Q07812
Optimal dilutions for each application to be determined by the researcher.
Antibody is Knockout validated.