ProSci

DOT1L Antibody

Product Code:
 
PSI-14-366
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using DOT1L antibody (14-366) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
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Western blot analysis of extracts of various cell lines, using DOT1L antibody (14-366) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.

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PSI-14-366-100uL100 uL£565.00
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Further Information

Additional Names:
DOT1, KMT4, histone-lysine N-methyltransferase, H3 lysine-79 specific, DOT1 like histone H3K79 methyltransferase, DOT1-like histone methyltransferase, DOT1-like protein, DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, histone H3-K79 methyltransferase, histone methyltransferase DOT1L, lysine N-methyltransferase 4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human DOT1L (NP_115871.1).
NCBI Gene ID #:
84444
NCBI Official Name:
DOT1 like histone lysine methyltransferase
NCBI Official Symbol:
DOT1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 170kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q8TEK3
User NOte:
Optimal dilutions for each application to be determined by the researcher.