ProSci

C1GALT1 Antibody

Product Code:
 
PSI-14-673
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of mouse kidney, using C1GALT1 antibody (14-673) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse kidney using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of mouse kidney, using C1GALT1 antibody (14-673) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using C1GALT1 antibody (14-673) at dilution of 1:100 (40x lens).

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PSI-14-673-100uL100 uL£565.00
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Further Information

Additional Names:
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, B3Gal-T8, Core 1 O-glycan T-synthase, Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1, Beta-1,3-galactosyltransferase, Core 1 beta1,3-galactosyltransferase 1, C1GalT1, Core 1 beta3-Gal-T1, C1GALT1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 194-363 of human C1GALT1 (NP_064541.1).
NCBI Gene ID #:
56913
NCBI Official Name:
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
NCBI Official Symbol:
C1GALT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9NS00
User NOte:
Optimal dilutions for each application to be determined by the researcher.