ProSci

DBI Antibody

Product Code:
 
PSI-14-894
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using DBI antibody (14-894) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human prostate using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse brain using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of MCF-7 cells using DBI antibody (14-894). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DBI antibody (14-894) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using DBI antibody (14-894). Blue: DAPI for nuclear staining.

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PSI-14-894-100uL100 uL£565.00
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Further Information

Additional Names:
DBI, ACBP, Acyl-CoA-binding protein, CCK-RP, Diazepam-binding inhibitor, ACBD1, GABA receptor modulator, EP, Endozepine
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human DBI (NP_001171513.1).
NCBI Gene ID #:
1622
NCBI Official Name:
diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein)
NCBI Official Symbol:
DBI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Obesity
Swissprot #:
P07108
User NOte:
Optimal dilutions for each application to be determined by the researcher.