MAP2K2 Antibody, KO Validated
Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
![Western blot analysis of extracts of HeLa cells, using MAP2K2 antibody (15-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s. Western blot analysis of extracts of HeLa cells, using MAP2K2 antibody (15-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](https://www.prosci-inc.com/static-images/15-718_1.jpg)
2 / 2
![Western blot analysis of extracts from normal (control) and MAP2K2 knockout (KO) 293T cells, using MAP2K2 antibody (15-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s. Western blot analysis of extracts from normal (control) and MAP2K2 knockout (KO) 293T cells, using MAP2K2 antibody (15-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.](https://www.prosci-inc.com/static-images/15-718_2.jpg)
❮❯No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC4
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K2 (NP_109587.1).
5605
mitogen-activated protein kinase kinase 2
MAP2K2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Affinity purification
Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
P36507
Optimal dilutions for each application to be determined by the researcher.
Antibody is Knockout validated.