ProSci

G3BP1 Antibody

Product Code:
 
PSI-15-756
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using G3BP1 antibody (15-756) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat liver using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using G3BP1 antibody (15-756) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using G3BP1 antibody (15-756) at dilution of 1:100 (40x lens).

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CodeSizePrice
PSI-15-756-100uL100 uL£565.00
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Further Information

Additional Names:
G3BP, G3BP, HDH-VIII
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_938405.1).
NCBI Gene ID #:
10146
NCBI Official Name:
GTPase activating protein (SH3 domain) binding protein 1
NCBI Official Symbol:
G3BP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13283
User NOte:
Optimal dilutions for each application to be determined by the researcher.