ProSci

CLCA1 Antibody

Product Code:
 
PSI-15-843
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using CLCA1 antibody (15-843) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
2 / 4
Immunofluorescence analysis of rat colon using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of human colon carcinoma using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of mouse colon using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CLCA1 antibody (15-843) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunofluorescence analysis of rat colon using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human colon carcinoma using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse colon using CLCA1 Polyclonal Antibody (15-843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-15-843-100uL100 uL£565.00
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Further Information

Additional Names:
CLCA1, chloride channel, calcium activated, family member 1, HGNC:2015, CaCC, calcium activated chloride channel 1, CACC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-150 of human CLCA1 (NP_001276.2).
NCBI Gene ID #:
1179
NCBI Official Name:
chloride channel accessory 1
NCBI Official Symbol:
CLCA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 85kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
A8K7I4
User NOte:
Optimal dilutions for each application to be determined by the researcher.