ProSci

FLII Antibody

Product Code:
 
PSI-15-954
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using FLII antibody (15-954) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5S.
2 / 4
Immunohistochemistry of paraffin-embedded rat heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human colon carcinoma using FLII antibody (15-954) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using FLII antibody (15-954) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5S.
Immunohistochemistry of paraffin-embedded rat heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FLII antibody (15-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).

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PSI-15-954-100uL100 uL£565.00
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Further Information

Additional Names:
FLI, FLIL, Fli1, protein flightless-1 homolog, flightless I actin binding protein, flightless I homolog
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
NCBI Gene ID #:
2314
NCBI Official Name:
FLII actin remodeling protein
NCBI Official Symbol:
FLII
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
Q13045
User NOte:
Optimal dilutions for each application to be determined by the researcher.