ProSci

RAPGEF1 Antibody

Product Code:
 
PSI-16-400
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunofluorescence analysis of L929 cells using RAPGEF1 Polyclonal Antibody (16-400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using RAPGEF1 Polyclonal Antibody (16-400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of L929 cells using RAPGEF1 Polyclonal Antibody (16-400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RAPGEF1 Polyclonal Antibody (16-400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-16-400-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
C3G, GRF2, rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Rap guanine nucleotide exchange factor (GEF) 1, guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1).
NCBI Gene ID #:
2889
NCBI Official Name:
Rap guanine nucleotide exchange factor 1
NCBI Official Symbol:
RAPGEF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q13905
User NOte:
Optimal dilutions for each application to be determined by the researcher.