ProSci

S100A8 Antibody

Product Code:
 
PSI-16-785
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using S100A8 antibody (16-785) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
2 / 3
Immunohistochemistry of paraffin-embedded human esophageal cancer using S100A8 antibody (16-785) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (16-785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using S100A8 antibody (16-785) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using S100A8 antibody (16-785) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (16-785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-16-785-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).
NCBI Gene ID #:
6279
NCBI Official Name:
S100 calcium binding protein A8
NCBI Official Symbol:
S100A8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity, Neuroscience, Signal Transduction
Swissprot #:
P05109
User NOte:
Optimal dilutions for each application to be determined by the researcher.