ProSci

PSMD10 Antibody

Product Code:
 
PSI-18-408
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of SW620 cells, using PSMD10 antibody (18-408) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse spleen using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of MCF7 cells using PSMD10 antibody (18-408). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of SW620 cells, using PSMD10 antibody (18-408) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using PSMD10 antibody (18-408) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using PSMD10 antibody (18-408). Blue: DAPI for nuclear staining.

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PSI-18-408-100uL100 uL£565.00
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Further Information

Additional Names:
26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, p28(GANK), PSMD10
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human PSMD10 (NP_002805.1).
NCBI Gene ID #:
5716
NCBI Official Name:
26S proteasome non-ATPase regulatory subunit 10
NCBI Official Symbol:
PSMD10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
O75832
User NOte:
Optimal dilutions for each application to be determined by the researcher.