ProSci

BAG5 Antibody

Product Code:
 
PSI-19-862
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using BAG5 antibody (19-862) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using BAG5 antibody (19-862) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat brain using BAG5 antibody (19-862) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using BAG5 antibody (19-862) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using BAG5 antibody (19-862) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BAG5 antibody (19-862) at dilution of 1:100 (40x lens).

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PSI-19-862-100uL100 uL£565.00
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Further Information

Additional Names:
BAG-family molecular chaperone regulator-5, BAG-5, BCL2-associated athanogene 5, BAG5, KIAA0873
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).
NCBI Gene ID #:
9529
NCBI Official Name:
BCL2-associated athanogene 5
NCBI Official Symbol:
BAG5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q9UL15
User NOte:
Optimal dilutions for each application to be determined by the researcher.