ProSci

HNRNPR Antibody

Product Code:
 
PSI-19-917
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HNRNPR antibody (19-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse liver using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using HNRNPR antibody (19-917) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HNRNPR antibody (19-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat heart using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HNRNPR antibody (19-917) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HNRNPR antibody (19-917) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-917-100uL100 uL£565.00
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Further Information

Additional Names:
HNRNPR, FLJ25714, HNRPR, hnRNP-R, hnRNP R
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human HNRNPR (NP_005817.1).
NCBI Gene ID #:
10236
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein R
NCBI Official Symbol:
HNRNPR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O43390
User NOte:
Optimal dilutions for each application to be determined by the researcher.