ProSci

DCTD Antibody

Product Code:
 
PSI-19-923
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using DCTD antibody (19-923) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using DCTD antibody (19-923) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse kidney using DCTD antibody (19-923) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using DCTD antibody (19-923) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using DCTD antibody (19-923) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DCTD antibody (19-923) at dilution of 1:200 (40x lens).

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PSI-19-923-100uL100 uL£565.00
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Further Information

Additional Names:
deoxycytidylate deaminase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human DCTD (NP_001912.2).
NCBI Gene ID #:
1635
NCBI Official Name:
dCMP deaminase
NCBI Official Symbol:
DCTD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P32321
User NOte:
Optimal dilutions for each application to be determined by the researcher.