ProSci

GLRX3 Antibody

Product Code:
 
PSI-19-926
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using GLRX3 antibody (19-926) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GLRX3 antibody (19-926). Western blot was performed from the immunoprecipitate using GLRX3 antibody (19-926) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using GLRX3 antibody (19-926) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GLRX3 antibody (19-926). Western blot was performed from the immunoprecipitate using GLRX3 antibody (19-926) at a dilition of 1:1000.

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PSI-19-926-100uL100 uL£565.00
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Further Information

Additional Names:
GLRX3, FLJ11864, GLRX4, GRX3, GRX4, PICOT, TXNL2, TXNL3, bA500G10.4
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GLRX3 (NP_001186797.1).
NCBI Gene ID #:
10539
NCBI Official Name:
glutaredoxin 3
NCBI Official Symbol:
GLRX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O76003
User NOte:
Optimal dilutions for each application to be determined by the researcher.