ProSci

ETF1 Antibody

Product Code:
 
PSI-19-944
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using ETF1 antibody (19-944) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
2 / 2
Immunofluorescence analysis of NIH-3T3 cells using ETF1 Polyclonal Antibody (19-944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ETF1 antibody (19-944) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunofluorescence analysis of NIH-3T3 cells using ETF1 Polyclonal Antibody (19-944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-19-944-100uL100 uL£565.00
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Further Information

Additional Names:
D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1, eukaryotic peptide chain release factor subunit 1, polypeptide chain release factor 1, protein Cl1, sup45 (yeast omnipotent suppressor 45) homolog-like 1
Application Note:
WB: 1:200 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1).
NCBI Gene ID #:
2107
NCBI Official Name:
eukaryotic translation termination factor 1
NCBI Official Symbol:
ETF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62495
User NOte:
Optimal dilutions for each application to be determined by the researcher.