ProSci

EIF3D Antibody

Product Code:
 
PSI-19-956
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using EIF3D antibody (19-956) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunofluorescence analysis of L-929 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 5
Immunofluorescence analysis of C6 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of L-929 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using EIF3D antibody (19-956) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of L-929 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (19-956) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-956-100uL100 uL£565.00
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Further Information

Additional Names:
EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta, EIF3S7
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).
NCBI Gene ID #:
8664
NCBI Official Name:
eukaryotic translation initiation factor 3, subunit D
NCBI Official Symbol:
EIF3D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O15371
User NOte:
Optimal dilutions for each application to be determined by the researcher.