ProSci

PRPF8 Antibody

Product Code:
 
PSI-19-988
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using PRPF8 antibody (19-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 5
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (19-988) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PRPF8 antibody (19-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (19-988) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.

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PSI-19-988-100uL100 uL£565.00
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Further Information

Additional Names:
HPRP8, PRP8, PRPC8, RP13, SNRNP22pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p22precursor mRNA processing protein, splicing factor Prp8
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
NCBI Gene ID #:
10594
NCBI Official Name:
pre-mRNA processing factor 8
NCBI Official Symbol:
PRPF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 274kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q6P2Q9
User NOte:
Optimal dilutions for each application to be determined by the researcher.