ProSci

COPS3 Antibody

Product Code:
 
PSI-22-634
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using COPS3 antibody (22-634) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of MCF7 cells using COPS3 antibody (22-634). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using COPS3 antibody (22-634) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of MCF7 cells using COPS3 antibody (22-634). Blue: DAPI for nuclear staining.

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PSI-22-634-100uL100 uL£565.00
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Further Information

Additional Names:
COPS3, SGN3, JAB1-containing signalosome subunit 3, CSN3, COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis), COP9 constitutive photomorphogenic homolog subunit 3, COP9 complex subunit 3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 194-423 of human COPS3 (NP_003644.2).
NCBI Gene ID #:
8533
NCBI Official Name:
COP9 signalosome subunit 3
NCBI Official Symbol:
COPS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q9UNS2
User NOte:
Optimal dilutions for each application to be determined by the researcher.