ProSci

mH2A1 Antibody

Product Code:
 
PSI-22-656
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using H2AFY antibody (22-656) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human kidney using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using mH2A1 antibody (22-656). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using H2AFY antibody (22-656) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using mH2A1 antibody (22-656). Blue: DAPI for nuclear staining.

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PSI-22-656-100uL100 uL£686.00
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Further Information

Additional Names:
H2AFY, H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2).
NCBI Gene ID #:
9555
NCBI Official Name:
H2A histone family, member Y
NCBI Official Symbol:
H2AFY
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75367
User NOte:
Optimal dilutions for each application to be determined by the researcher.