ProSci

NCOR1 Antibody

Product Code:
 
PSI-22-657
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using NCOR1 antibody (22-657) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using NCOR1 antibody (22-657). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using NCOR1 antibody (22-657) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using NCOR1 antibody (22-657). Blue: DAPI for nuclear staining.

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PSI-22-657-100uL100 uL£565.00
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Further Information

Additional Names:
NCOR1, N-CoR, TRAC1, N-CoR1, hN-CoR
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NCOR1 (NP_006302.2).
NCBI Gene ID #:
9611
NCBI Official Name:
nuclear receptor corepressor 1
NCBI Official Symbol:
NCOR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 320kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
O75376
User NOte:
Optimal dilutions for each application to be determined by the researcher.