ProSci

BCAP31 Antibody

Product Code:
 
PSI-22-664
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using BCAP31 antibody (22-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of U2OS cells using BCAP31 antibody (22-664). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAP31 antibody (22-664). Western blot was performed from the immunoprecipitate using BCAP31 antibody (22-664) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using BCAP31 antibody (22-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using BCAP31 antibody (22-664). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAP31 antibody (22-664). Western blot was performed from the immunoprecipitate using BCAP31 antibody (22-664) at a dilition of 1:1000.

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PSI-22-664-100uL100 uL£565.00
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Further Information

Additional Names:
BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-246 of human BCAP31 (NP_001243376.1).
NCBI Gene ID #:
10134
NCBI Official Name:
B-cell receptor-associated protein 31
NCBI Official Symbol:
BCAP31
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
P51572
User NOte:
Optimal dilutions for each application to be determined by the researcher.