ProSci

BNIP1 Antibody

Product Code:
 
PSI-22-821
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using BNIP1 Antibody (22-821) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded rat brain using BNIP1 Antibody (22-821) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using BNIP1 Antibody (22-821) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using BNIP1 Antibody (22-821) at dilution of 1:100 (40x lens).

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PSI-22-821-100uL100 uL£565.00
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Further Information

Additional Names:
BNIP1, BCL2/adenovirus E1B 19 kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1, SEC20L, TRG8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2).
NCBI Gene ID #:
662
NCBI Official Name:
BCL2/adenovirus E1B 19kDa interacting protein 1
NCBI Official Symbol:
BNIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q12981
User NOte:
Optimal dilutions for each application to be determined by the researcher.