ProSci

SH3GL1 Antibody

Product Code:
 
PSI-22-949
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of COS-7 cells, using SH3GL1 antibody (22-949) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Western blot analysis of extracts of COS-7 cells, using SH3GL1 antibody (22-949) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

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CodeSizePrice
PSI-22-949-100uL100 uL£565.00
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Further Information

Additional Names:
extra eleven-nineteen leukemia fusion gene protein, extra 11-19 leukemia fusion, SH3 domain protein 2B, endophilin-2, endophilin-A2, SH3-containing protein EEN, SH3-containing Grb-2-like 1 protein, SH3 domain-containing GRB2-like protein 1, SH3 domain GRB2-like 1, EEN fusion partner of MLL, SH3P8, SH3D2B, MGC111371, EEN, CNSA1, SH3-domain GRB2-like 1, SH3GL1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 219-368 of human SH3GL1 (NP_003016.1).
NCBI Gene ID #:
6455
NCBI Official Name:
SH3-domain GRB2-like 1
NCBI Official Symbol:
SH3GL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q99961
User NOte:
Optimal dilutions for each application to be determined by the researcher.