ProSci

RRAGC Antibody

Product Code:
 
PSI-22-971
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using RRAGC antibody (22-971) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded rat intestine using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded rat brain using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse intestine using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A549 cells using RRAGC antibody (22-971).
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Western blot analysis of extracts of various cell lines, using RRAGC antibody (22-971) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat intestine using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestine using RRAGC Antibody (22-971) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using RRAGC antibody (22-971).

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PSI-22-971-100uL100 uL£565.00
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Further Information

Additional Names:
Ras-related GTP-binding protein C, Rag C, RagC, GTPase-interacting protein 2, TIB929, RRAGC (HGNC:19902)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the GTR/RAG GTP-binding protein family. The encoded protein is a monomeric guanine nucleotide-binding protein which forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. The encoded protein promotes intracellular localization of the mTOR complex. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RRAGC (NP_071440.1).
NCBI Gene ID #:
64121
NCBI Official Name:
Ras-related GTP-binding protein C
NCBI Official Symbol:
RRAGC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cell Cycle, Signal Transduction
Swissprot #:
Q9HB90
User NOte:
Optimal dilutions for each application to be determined by the researcher.