ProSci

PPP4R1 Antibody

Product Code:
 
PSI-23-534
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (23-534) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (23-534) at dilution of 1:100 (40x lens).
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (23-534). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (23-534) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (23-534) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (23-534) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (23-534). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (23-534) at a dilition of 1:1000.

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PSI-23-534-100uL100 uL£565.00
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Further Information

Additional Names:
MEG1, PP4(Rmeg), PP4R1, serine/threonine-protein phosphatase 4 regulatory subunit 1, serine/threonine phosphatase 4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
NCBI Gene ID #:
9989
NCBI Official Name:
protein phosphatase 4 regulatory subunit 1
NCBI Official Symbol:
PPP4R1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q8TF05
User NOte:
Optimal dilutions for each application to be determined by the researcher.