ProSci

TMEM189 Antibody

Product Code:
 
PSI-23-544
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (23-544) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody (23-544) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (23-544) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody (23-544) at dilution of 1:100 (40x lens).

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PSI-23-544-100uL100 uL£565.00
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Further Information

Additional Names:
KUA, transmembrane protein 189
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
Co-transcription of this gene and the neighboring downstream gene (ubiquitin-conjugating enzyme E2 variant 1) generates a rare read-through transcript, which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. The protein encoded by this individual gene lacks a UEV1 domain but includes three transmembrane regions. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human TMEM189 (NP_954580.1).
NCBI Gene ID #:
387521
NCBI Official Name:
transmembrane protein 189
NCBI Official Symbol:
TMEM189
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
A5PLL7
User NOte:
Optimal dilutions for each application to be determined by the researcher.