ProSci

CD163 Antibody

Product Code:
 
PSI-23-546
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CD163 Antibody (23-546) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded Rat lung using CD163 antibody (23-546) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using CD163 Antibody (23-546) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded Rat lung using CD163 antibody (23-546) at dilution of 1:100 (40x lens).

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PSI-23-546-100uL100 uL£565.00
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Further Information

Additional Names:
CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 molecule, Hemoglobin scavenger receptor, MM130
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:100 - 1:200
Background:
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 750-1050 of human CD163 (NP_004235.4).
NCBI Gene ID #:
9332
NCBI Official Name:
CD163 molecule
NCBI Official Symbol:
CD163
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 180kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity
Swissprot #:
Q86VB7
User NOte:
Optimal dilutions for each application to be determined by the researcher.