ProSci

RNASEL Antibody

Product Code:
 
PSI-23-936
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using RNASEL antibody (23-936) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human lung cancer using RNASEL antibody (23-936) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RNASEL antibody (23-936) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using RNASEL antibody (23-936) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using RNASEL antibody (23-936) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNASEL antibody (23-936) at dilution of 1:100 (40x lens).

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PSI-23-936-100uL100 uL£565.00
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Further Information

Additional Names:
RNASEL, RNS4, PRCA1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1).
NCBI Gene ID #:
6041
NCBI Official Name:
ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
NCBI Official Symbol:
RNASEL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 84kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
Q05823
User NOte:
Optimal dilutions for each application to be determined by the researcher.