ProSci

WDR77 Antibody [8B2F7]

Product Code:
 
PSI-23-974
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[8B2F7]
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using WDR77 antibody (23-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using WDR77 antibody (23-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-23-974-100uL100 uL£686.00
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Further Information

Additional Names:
WDR77, Androgen receptor cofactor p44, HKMT1069, MEP-50, Methylosome protein 50, p44, MEP50, Nbla10071, RP11-552M11.3, WD repeat domain 77
Application Note:
WB: 1:500 - 1:2000

ICC: 1:50 - 1:200
Background:
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human WDR77
NCBI Gene ID #:
79084
NCBI Official Name:
WD repeat domain 77
NCBI Official Symbol:
WDR77
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9BQA1
User NOte:
Optimal dilutions for each application to be determined by the researcher.