RevMab Biosciences

anti-His-Tag Chimeric Human Rabbit Monoclonal (RMH01)

Product Code:
 
REV-54-1161-00
Product Group:
 
Primary Antibodies
Supplier:
 
RevMab Biosciences
Antibody Isotype:
 
Chimeric
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RMH01
Regulatory Status:
 
RUO
Target Species:
 
Universal
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
+4°C
1 / 1
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antib
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Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using chimeric human anti-HisTag antibody Clone RMH01 at 0.2ug/mL, followed by a HRP conjugated anti-Human IgG secondary antib

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REV-54-1161-00-C100100 ug£485.00
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This product comes from: Switzerland.
Typical lead time: 14-21 working days.
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Further Information

Alternate Names/Synonyms:
Polyhistidin
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Long Description:
Recombinant Antibody. This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody. Applications: WB, ELISA. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein?s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
NCBI, Uniprot Number:
N/A
Package Type:
Vial
Product Description:
His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein?s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
Purity:
Protein A purified.
Source / Host:
Rabbit
Specificity:
This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.