SARS-CoV-2 (COVID-19) Nucleocapsid Antibody
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.
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![<strong>Figure 2 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br>
SARS-CoV N protein (Lane 1: 50ng, Lane 2: 25ng, Lane 3: 15ng, Lane 4: 10ng , Lane 5: 5ng, Lane 6: 2.5ng) and SARS-CoV-2 N protein (Lane 7: 50ng, Lane 8: 25ng, Lane 9: 15ng, Lane 10: 10ng , Lane 11: 5ng, Lane 12: 2.5ng) probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 10-610, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C. <strong>Figure 2 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br>
SARS-CoV N protein (Lane 1: 50ng, Lane 2: 25ng, Lane 3: 15ng, Lane 4: 10ng , Lane 5: 5ng, Lane 6: 2.5ng) and SARS-CoV-2 N protein (Lane 7: 50ng, Lane 8: 25ng, Lane 9: 15ng, Lane 10: 10ng , Lane 11: 5ng, Lane 12: 2.5ng) probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 10-610, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C.](https://www.prosci-inc.com/static-images/SARS-CoV-2-COVID-19-Nucleocapsid-Antibody_WB-2_10-610.jpg)
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![<strong>Figure 1 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br>
Lane 1: 50ng, Lane 2: 25ng, Lane 3: 15ng, Lane 4: 10ng , Lane 5: 5ng and Lane 6: 2.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 10-610, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C. <strong>Figure 1 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br>
Lane 1: 50ng, Lane 2: 25ng, Lane 3: 15ng, Lane 4: 10ng , Lane 5: 5ng and Lane 6: 2.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 10-610, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C.](https://www.prosci-inc.com/static-images/SARS-CoV-2-COVID-19-Nucleocapsid-Antibody_WB_10-610.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus N Protein, novel coronavirus Nucleocapsid Protein, 2019-nCoV Nucleoprotein, 2019-nCoV N, 2019nCoV N, 2019-nCoV N Protein, 2019 peak N Protein, 2019-nCoV nucleocapsid protein.
WB(1:500-1000), ELISA(1:5000-10000)
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
0.01M PBS(pH7.4) with 0.1% Proclin300
?1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant SARS-CoV-2 Nucleocapsid
Nucleocapsid phosphoprotein
N
SARS-CoV-2
Liquid
Purified by Protein A.
Infectious Disease,COVID-19
Optimal dilutions for each application to be determined by the researcher.