NSJ Bioreagents

Anti-AKT2 Antibody

Product Code:
 
NSJ-F40226
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
148CT5.4.1.4
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the anti-AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Anti-AKT2 antibody western blot analysis in mouse brain tissue lysate
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Anti-AKT2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.
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Confocal immunofluorescent analysis of anti-AKT2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
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Anti-AKT2 antibody western blot analysis in mouse brain tissue lysate
Anti-AKT2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.
Confocal immunofluorescent analysis of anti-AKT2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

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CodeSizePrice
NSJ-F40226-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40226-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:100-1:500,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the anti-AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This anti-AKT2 antibody was produced from a mouse immunized with AKT2 recombinant protein.
Limitation:
This anti-AKT2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P31751