NSJ Bioreagents

GAPDH Antibody

Product Code:
 
NSJ-F40186
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1A10A10
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 6
GAPDH antibody WB at 1:1000 dilution. Lane 1: A431 lysate; 2: rat C6; 3: HeLa; 4: HUVEC; Predicted band size : 36 kDa.
2 / 6
Western blot testing of GAPDH antibody at 1:8000 dilution. Lane 1: HeLa lysate; 2: A549; 3: COS-7; 4: mouse brain; 5: rat C6; 6: NIH3T3; Predicted band size : 36 kDa.
3 / 6
Fluorescent image of HeLa cells stained with XAF1 GAPDH antibody. Ab was diluted at 1:25 dilution; Alexa Fluor 488-conjugated secondary Ab (green); Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
4 / 6
Immunohistochemical analysis of paraffin-embedded humankidney section using GAPDH antibody. Ab was diluted at 1:25 dilution.
5 / 6
Immunohistochemical analysis of paraffin-embedded human stomach section using GAPDH antibody
6 / 6
Western blot analysis of anti-GAPDH antibody and CEM lysate
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GAPDH antibody WB at 1:1000 dilution. Lane 1: A431 lysate; 2: rat C6; 3: HeLa; 4: HUVEC; Predicted band size : 36 kDa.
Western blot testing of GAPDH antibody at 1:8000 dilution. Lane 1: HeLa lysate; 2: A549; 3: COS-7; 4: mouse brain; 5: rat C6; 6: NIH3T3; Predicted band size : 36 kDa.
Fluorescent image of HeLa cells stained with XAF1 GAPDH antibody. Ab was diluted at 1:25 dilution; Alexa Fluor 488-conjugated secondary Ab (green); Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded humankidney section using GAPDH antibody. Ab was diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded human stomach section using GAPDH antibody
Western blot analysis of anti-GAPDH antibody and CEM lysate

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CodeSizePrice
NSJ-F40186-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40186-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000-8000,Immunofluorescence: 1:25,IHC (Paraffin): 1:25
Application Note:
Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
recombinant protein was used to produce this monoclonal GAPDH antibody.
Limitation:
This GAPDH antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04406