LIG1 Antibody / DNA Ligase 1

Western blot: 0.5-1ug/ml,ELISA,Immunoprecipitation

Optimal dilution of the antibody should be determined by the researcher.

DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Purified

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Full-length native cow LIG1 protein was used as the immunogen for the DNA Ligase 1 antibody.

This DNA Ligase 1 antibody is available for research use only.

Nuclear

Protein G affinity chromatography

Human, Mouse, Rat

P18858