NSJ Bioreagents

Ki67 antibody

Product Code:
 
NSJ-F54499
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2179CT8.1.2.1
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Ki67 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 3
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 3
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with Ki67 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54499-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54499-0.2ML0.2 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Ki67 antibody.
Limitation:
This Ki67 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P46013