NSJ Bioreagents

CD63 Antibody / LAMP-3

Product Code:
 
NSJ-V3029
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LAMP3/968
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the LAMP-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Flow cytometry testing of human MCF7 cells with LAMP-3 antibody (clone LAMP3/968); Gray=unstained, Orange= CF555-labeled LAMP-3 antibody.
2 / 4
FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Orange = CF555-labeled LAMP-3 antibody.
3 / 4
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with CD63 / LAMP-3 antibody (LAMP3/968).
4 / 4
SDS-PAGE Analysis of Purified, BSA-Free LAMP-3 Antibody (clone LAMP3/968). Confirmation of Integrity and Purity of the Antibody.

Flow cytometry testing of human MCF7 cells with LAMP-3 antibody (clone LAMP3/968); Gray=unstained, Orange= CF555-labeled LAMP-3 antibody.
FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Orange = CF555-labeled LAMP-3 antibody.
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with CD63 / LAMP-3 antibody (LAMP3/968).
SDS-PAGE Analysis of Purified, BSA-Free LAMP-3 Antibody (clone LAMP3/968). Confirmation of Integrity and Purity of the Antibody.

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CodeSizePrice
NSJ-V3029-20UG20 ug£264.00
Quantity:
NSJ-V3029-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the LAMP-3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP-3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human full-length protein was used as the immunogen for the CD63 / LAMP-3 antibody.
Limitation:
This LAMP-3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08962