NSJ Bioreagents

CDw75 Antibody

Product Code:
 
NSJ-V2855
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgM, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LN1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CDw75 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC analysis of formalin-fixed, paraffin-embedded normal human spleen stained with CDw75 antibody (clone LN1).~
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Surface staining of human lymphocytes with CDw75 antibody (clone LN1, red) and isotype control (green). PPI negative populati
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IHC analysis of formalin-fixed, paraffin-embedded normal human spleen stained with CDw75 antibody (clone LN1).~
Surface staining of human lymphocytes with CDw75 antibody (clone LN1, red) and isotype control (green). PPI negative populati

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CodeSizePrice
NSJ-V2855-20UG20 ug£264.00
Quantity:
NSJ-V2855-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CDw75 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Description:
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This mAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes were used as the immunogen for the CDw75 antibody.
Limitation:
This CDw75 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
PEG precipitation
Species Reactivity :
Human
Uniprot #:
P15907