NSJ Bioreagents

Secretory Component p85 Antibody / ECM1

Product Code:
 
NSJ-V2484
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rECM1/792
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Secretory Component Glycoprotein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant Secretory Component Glycoprotein antibody (rECM1/792).~
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SDS-PAGE Analysis of Purified, BSA-Free recombinant Secretory Component Glycoprotein Antibody (

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant Secretory Component Glycoprotein antibody (rECM1/792).~
SDS-PAGE Analysis of Purified, BSA-Free recombinant Secretory Component Glycoprotein Antibody (

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CodeSizePrice
NSJ-V2484-20UG20 ug£264.00
Quantity:
NSJ-V2484-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Application Note:
Optimal dilution of the recombinant Secretory Component Glycoprotein antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant Secretory Component Glycoprotein antibody.
Limitation:
This recombinant Secretory Component Glycoprotein antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q16610