NSJ Bioreagents

CD22 Antibody

Product Code:
 
NSJ-V8228
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RFB4
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Fluorescence-activated cell sorting (FACS)
Storage:
 
Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Flow cytometry testing of human Ramos cells with CD22 antibody (clone RFB4); Red=isotype control, Blue= CD22 antibody.~
2 / 2
SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone RFB4) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of human Ramos cells with CD22 antibody (clone RFB4); Red=isotype control, Blue= CD22 antibody.~
SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone RFB4) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8228-20UG20 ug£264.00
Quantity:
NSJ-V8228-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Human tonsil lymphocytes were used as the immunogen for the CD22 antibody.
Limitation:
This CD22 antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20273