NSJ Bioreagents

CD20 Antibody

Product Code:
 
NSJ-V7239
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RMC20-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
1 / 2
IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.~
2 / 2
Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone RMC20-1); Red=isotyp

IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.~
Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone RMC20-1); Red=isotyp

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CodeSizePrice
NSJ-V7239-20UG20 ug£264.00
Quantity:
NSJ-V7239-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Limitation:
This recombinant CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P11836