NSJ Bioreagents

Mitochondrial Marker Antibody

Product Code:
 
NSJ-V3079
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM198
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-Mitochondria antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondria antibody (SPM198).~
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Immunofluorescent staining of fixed human HeLa cells with anti-Mitochondria antibody (clone SPM198, green) and Reddot nuclear stain.
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Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondria antibody (SPM198).~
Immunofluorescent staining of fixed human HeLa cells with anti-Mitochondria antibody (clone SPM198, green) and Reddot nuclear stain.

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CodeSizePrice
NSJ-V3079-20UG20 ug£264.00
Quantity:
NSJ-V3079-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.
Limitation:
This anti-Mitochondria antibody is available for research use only.
Localization:
Cytoplasmic (Mitochondria)
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Not Known