NSJ Bioreagents

Aurora-C Antibody

Product Code:
 
NSJ-F50011
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the Aurora-C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Immunofluorescence staining of HeLa cells expressing GFP-Aurora-C performed at different cellular mitotic stages with the A) Aurora-C antibody, B) GFP fluorescence, C) DAPI nuclear staining, and D) anti-Aurora C merged to DAPI staining.
2 / 3
Western blot testing of Aurora-C antibody and lysate of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora-A (2), -B (3), -C (4).
3 / 3
Aurora-C antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~33 kDa.
❮❯

Immunofluorescence staining of HeLa cells expressing GFP-Aurora-C performed at different cellular mitotic stages with the A) Aurora-C antibody, B) GFP fluorescence, C) DAPI nuclear staining, and D) anti-Aurora C merged to DAPI staining.
Western blot testing of Aurora-C antibody and lysate of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora-A (2), -B (3), -C (4).
Aurora-C antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~33 kDa.

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CodeSizePrice
NSJ-F50011-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50011-0.4ML0.4 ml£502.00
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Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the Aurora-C antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 3-38 from the human protein was used as the immunogen for this Aurora-C antibody.
Limitation:
This Aurora-C antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9UQB9