NSJ Bioreagents

IL-1B Antibody

Product Code:
 
NSJ-F51185
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the IL-1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Flow cytometric analysis of NCI-H292 cells using IL-1B antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 3
IHC analysis of FFPE human lung carcinoma with IL-1B antibody
3 / 3
Western blot analysis of IL-1B antibody and NCI-H460 lysate. Predicted molecular weight: 31 kDa (precursor) and 17 kDa (mature).
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Flow cytometric analysis of NCI-H292 cells using IL-1B antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human lung carcinoma with IL-1B antibody
Western blot analysis of IL-1B antibody and NCI-H460 lysate. Predicted molecular weight: 31 kDa (precursor) and 17 kDa (mature).

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CodeSizePrice
NSJ-F51185-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51185-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the IL-1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IL1B is produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 148-174 from the human protein was used as the immunogen for this IL-1B antibody.
Limitation:
This IL-1B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Primate, Rabbit
Uniprot #:
P01584