NSJ Bioreagents

AMPK alpha 2 Antibody

Product Code:
 
NSJ-F50381
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the AMPK alpha 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot analysis of AMPK alpha 2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (2).~
2 / 2
IHC analysis of FFPE human skeletal muscle stained with AMPK alpha 2 an
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Western blot analysis of AMPK alpha 2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (2).~
IHC analysis of FFPE human skeletal muscle stained with AMPK alpha 2 an

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CodeSizePrice
NSJ-F50381-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50381-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the AMPK alpha 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 453-483 from the human protein was used as the immunogen for this AMPK alpha 2 antibody.
Limitation:
This AMPK alpha 2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P54646