ARF1 Antibody

Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Titration of the ARF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

ARF1 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). [UniProt]

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 80-106 from the human protein was used as the immunogen for this ARF1 antibody.

This ARF1 antibody is available for research use only.

Antigen affinity

Human

Mouse, Rat, Bovine, Primate, Xenopus

P84077