NSJ Bioreagents

NEU2 Antibody

Product Code:
 
NSJ-F41977
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the NEU2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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NEU2 antibody western blot analysis in A549 lysate. Predicted molecular weight ~42 kDa.
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NEU2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue.
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Confocal immunofluorescent analysis of NEU2 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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NEU2 antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot testing of human fetal liver tissue lysate with NEU2 antibody. Predicted molecular weight ~42 kDa.
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Western blot testing of human heart tissue lysate with NEU2 antibody. Predicted molecular weight ~42 kDa.
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IHC testing of FFPE human skeletal muscle tissue with NEU2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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NEU2 antibody western blot analysis in A549 lysate. Predicted molecular weight ~42 kDa.
NEU2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue.
Confocal immunofluorescent analysis of NEU2 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
NEU2 antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of human fetal liver tissue lysate with NEU2 antibody. Predicted molecular weight ~42 kDa.
Western blot testing of human heart tissue lysate with NEU2 antibody. Predicted molecular weight ~42 kDa.
IHC testing of FFPE human skeletal muscle tissue with NEU2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F41977-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41977-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the NEU2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 23-50 from the human protein was used as the immunogen for this NEU2 antibody.
Limitation:
This NEU2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9Y3R4