NSJ Bioreagents

AKT2 Antibody

Product Code:
 
NSJ-F50043
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Fluorescent confocal image of C2C12 cell stained with AKT2 antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
2 / 3
Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (2).
3 / 3
IHC analysis of FFPE human lung carcinoma tissue stained with AKT2 antibody
❮❯

Fluorescent confocal image of C2C12 cell stained with AKT2 antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
Western blot analysis of AKT2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (2).
IHC analysis of FFPE human lung carcinoma tissue stained with AKT2 antibody

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CodeSizePrice
NSJ-F50043-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50043-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 452-481 from the human protein was used as the immunogen for this AKT2 antibody.
Limitation:
This AKT2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P31751