NSJ Bioreagents

ATG-5 Antibody

Product Code:
 
NSJ-F46220
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the ATG-5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot analysis of ATG-5 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the human gene. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
2 / 5
Western blot analysis of lysate from K562 cell line using ATG-5 antibody at 1:1000. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
3 / 5
ATG-5 antibody western blot analysis in uterus tumor lysate. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
4 / 5
Fluorescent image of U251 cells stained with ATG-5 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
5 / 5
ATG-5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.
❮❯

Western blot analysis of ATG-5 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the human gene. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
Western blot analysis of lysate from K562 cell line using ATG-5 antibody at 1:1000. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
ATG-5 antibody western blot analysis in uterus tumor lysate. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
Fluorescent image of U251 cells stained with ATG-5 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
ATG-5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F46220-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46220-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500,Immunofluorescence: 1:200,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the ATG-5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ATG-5 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5.
Limitation:
This ATG-5 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9H1Y0