NSJ Bioreagents

Beta III Tubulin Antibody (TUBB3)

Product Code:
 
NSJ-F49314
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the beta III Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Beta III Tubulin antibody western blot analysis in HepG2 lysate
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Beta III Tubulin antibody western blot analysis in mouse brain tissue lysate.
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Beta III Tubulin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
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Beta III Tubulin antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Beta III Tubulin antibody western blot analysis in HepG2 lysate
Beta III Tubulin antibody western blot analysis in mouse brain tissue lysate.
Beta III Tubulin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
Beta III Tubulin antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F49314-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49314-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the beta III Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 36-63 from the human protein was used as the immunogen for this beta III Tubulin antibody.
Limitation:
This beta III Tubulin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine, Primate, Chicken
Uniprot #:
Q13509